Anti-Human CD19 In Vivo Antibody

Product Name:	Anti-Human CD19 In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0328
Size:	1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:	SJ25-C1
Protein:	CD19
Product Type:	Monoclonal Antibody
Synonyms:	CD19 Molecule, Differentiation Antigen CD19, B-Lymphocytes Surface Antigen B4, T-cell Surface Antigen Leu-12, CVID3, B4, B-lymphocyte Antigen CD19
Isotype:	IgG1 k
Reactivity:	Human
Applications:	ELISA, FC, In Vivo
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC
Product Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Applications:	ELISA, FC, In Vivo
Reactivity:	Human

Host Species:	Mouse
Specificity:	Clone SJ25-C1 recognizes an epitope on human CD19.
Antigen Distribution:	CD19 is expressed in the majority of Pro-B cells to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19.
Immunogen:	NALM1 + NALM16 cells
Concentration:	≥ 2.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:	≥95% monomer by analytical SEC
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	This antibody is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

CD19 is a 95 kD transmembrane glycoprotein and member of the Ig superfamily. The antigen serves as an adaptor protein; drawing cytoplasmic signaling proteins to the membrane. It works via the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. Because of its presence on all B cells, CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for the immunotherapy of lymphoproliferative disorders. Emerging studies indicate that CD19 plays an active role in fueling the growth of these cancers, most notably by stabilizing the concentrations of the MYC oncoprotein, making CD19 an attractive therapeutic target with respect to its downstream signaling.

Protein:	CD19
Function:	B cell activation and differentiation
Ligand/Receptor:	Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Research Area:	Costimulatory Molecules, Immunology
Shipping:	2-8°C
NCBI Gene Bank ID:	930
UniProt:	CD19