Description
Anti-Mouse CD62L In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD62L In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD62L (L-selectin) protein, which is essential for lymphocyte trafficking and homing, making it ideal for research in immunology and cell migration. With a mouse IgG1 isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. CD62L is a cell adhesion molecule found on the surface of leukocytes, including lymphocytes, neutrophils, monocytes, and eosinophils. It plays a significant role in the immune system by mediating the initial capture and rolling of leukocytes on vascular endothelium, which is critical for leukocyte extravasation and migration to sites of inflammation or tissue injury.
| Product Name: | Anti-Mouse CD62L In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0064 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | MEL-14 |
| Protein: | CD62L |
| Product Type: | Monoclonal Antibody |
| Synonyms: | L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14 |
| Isotype: | Rat IgG2a κ |
| Reactivity: | Mouse |
| Immunogen: | C3H/eb mouse B lymphoma 38C-13 |
| Applications: | B, CyTOF®, FA, FC, IHC FF, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CyTOF®, FA, FC, IHC FF, IP, WB |
| Recommended Usage: | FC The suggested concentration for this MEL-14 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this MEL-14 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Rat Anti-Mouse CD62L (Clone MEL-14) recognizes an epitope on Mouse CD62L. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| Antigen Distribution: | Subsets of B and T cells, monocytes, granulocytes, subset of NK cells |
| Immunogen: | C3H/eb mouse B lymphoma 38C-13 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD62L is a 74-95 kD glycoprotein is a member of the selectin family and is expressed on the majority of B and naive T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. Furthermore, CD62L is a cell adhesion molecule that binds to many glycoprotein ligands including CD34, GlyCAM-1, and PSGL-1. Clone MEL-14 is reported as a Nutralization antibody.
| Datasheet: | View |
| Protein: | CD62L |
| Function: | Lymphocyte homing to HEV, rolling on activated endothelium |
| Ligand/Receptor: | CD34, GlyCAM-1, MAdCAM-1 |
| Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity |
