Anti-Mouse NK1.1 In Vivo Antibody

Product Name:	Anti-Mouse NK1.1 In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0100
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	PK136
Protein:	NK1.1
Product Type:	Monoclonal Antibody
Synonyms:	Natural Killer Cells, KR-P1C, NKR-P1B, Ly-55, CD161b, CD161c
Isotype:	Mouse IgG2a κ
Reactivity:	Mouse
Immunogen:	NK-1+ cells from mouse spleen and bone marrow
Applications:	B, CyTOF®, Depletion, FC, IP, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0100	Anti-Mouse NK1.1 In Vivo Antibody - Low Endotoxin	Low
IVMB0101	Anti-Mouse NK1.1 In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	B, CyTOF®, Depletion, FC, IP, WB
Recommended Usage:	FC The suggested use of this antibody is ≤ 0.25 µg per 10⁶ in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Reactivity:	Mouse

Host Species:	Mouse
Specificity:	Clone PK136 recognizes mouse NK1.1.
Antigen Distribution:	NK-1.1 is encoded by the NKR-P1B/NKR-P1C gene and expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129.
Immunogen:	NK-1+ cells from mouse spleen and bone marrow
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

NK1.1 is a type II membrane protein that is part of the C-type lectin superfamily. NK1.1 contains a transmembrane domain and a cytoplasmic domain that are characteristic of C-type lectins. The function of NK cells is to mediate cytotoxicity and to secrete cytokines after immune stimulation. NK1.1 has been correlated with lysis of tumor cells In vitro and rejection of bone marrow allografts In vivo. It is also involved in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 is commonly used as a mouse NK cell marker.

Technical Datasheet:	View
Protein:	NK1.1
Function:	NK cell activation, IFN-γ production, cytotoxic granule release
Research Area:	Immunology, Innate Immunity

Mouse IgG2a Isotype Control
Clone	C1.18.4
Isotype	Mouse IgG2a
Endotoxin Level	Low Endotoxin

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