Anti-Mouse CD62L In Vivo Antibody

Product Name:	Anti-Mouse CD62L In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0065
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	MEL-14
Protein:	CD62L
Product Type:	Monoclonal Antibody
Synonyms:	L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Isotype:	Rat IgG2a κ
Reactivity:	Mouse
Immunogen:	C3H/eb mouse B lymphoma 38C-13
Applications:	B, CyTOF®, FC, IHC FF, IP, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0064	Anti-Mouse CD62L In Vivo Antibody - Low Endotoxin	Low
IVMB0065	Anti-Mouse CD62L In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	B, CyTOF®, FC, IHC FF, IP, WB
Recommended Usage:	FC The suggested concentration for this MEL-14 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 10⁶ in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this MEL-14 antibody for use in western blotting is 1-10 µg/ml.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Rat Anti-Mouse CD62L (Clone MEL-14) recognizes an epitope on Mouse CD62L. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:	Subsets of B and T cells, monocytes, granulocytes, subset of NK cells
Immunogen:	C3H/eb mouse B lymphoma 38C-13
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD62L is a 74-95 kD glycoprotein is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. Furthermore, CD62L is a cell adhesion molecule that binds to many glycoprotein ligands including CD34, GlyCAM-1, and PSGL-1. Clone MEL-14 is reported as a Nutralization antibody.

Technical Datasheet:	View
Protein:	CD62L
Function:	Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor:	CD34, GlyCAM-1, MAdCAM-1
Research Area:	Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity

Rat IgG2a Isotype Control
Clone	1-1
Isotype	Rat IgG2a
Endotoxin Level	Ultra Low Endotoxin

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