Western blot analysis of extracts of Recombinant Human IFNA1 Protein, using Interferon alpha 1 (IFN-α1) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
The Interferon alpha 1 (IFN-alpha1) Monoclonal Antibody (CAB21081) is a high-quality antibody developed for reliable detection and analysis of target proteins. Interferon alpha 1 is a key cytokine involved in antiviral responses and immune regulation. This polyclonal antibody, produced in rabbits, is highly specific and reactive with human samples. It has been validated for use in Western blot applications, allowing for the detection and analysis of IFN-alpha1 protein levels in various cell types.IFN-alpha1 plays a crucial role in modulating immune responses and has been implicated in various disease states, including viral infections and autoimmune disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. This cytokine is upregulated in preeclamptic placentas and is thought to be a mediator of preeclampsia.
Purification Method
Affinity purification
Gene ID
3439
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of Recombinant Human IFN-alpha 1/13(Q114A) Protein (RP00011), using Interferon alpha 1 (IFN-α1) Rabbit mAb (CAB21081) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
