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[KO Validated] ITCH Rabbit Monoclonal Antibody (CAB8624)

SKU:
CAB8624
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
ADMFD
AIF4
AIP4
NAPP1
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] ITCH Rabbit Monoclonal Antibody
SKU:CAB8624
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ITCH (Q96J02).
Sequence:MSDS GSQL GSMG SLTM KSQL QITV ISAK LKEN KKNW FGPS PYVE VTVD GQSK KTEK CNNT NSPK WKQP LTVI VTPV SKLH FRVW SHQT LKSD VLLG TAAL
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:AIF4; AIP4; ADMFD; NAPP1; CH
Positive Sample:HeLa,293T,Raji,NIH/3T3,C6,Mouse lung
Conjugate:Unconjugated
Cellular Localization:cell cortex, cytoplasm, cytoplasmic vesicle, cytosol, extracellular exosome, nucleoplasm, plasma membrane
Calculated MW:103kDa
Observed MW:103kDa

This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Purification Method:Affinity purification
Gene ID:83737
Clone Number:ARC1759
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using ITCH Rabbit mAb (CAB8624) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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