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Anti-LDL Receptor (LDLR) Antibody (CAB20808)

SKU:
CAB20808
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Goat
Isotype:
IgG
  • Immunofluorescence analysis of HepG2 cells using LDL Receptor (LDLR) Rabbit mAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of A-431 cells, using LDL Receptor (LDLR) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of HeLa cells using LDL Receptor (LDLR) Rabbit mAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using LDL Receptor (LDLR) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
€179 - €469
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Description

Antibody Name:Anti-LDL Receptor (LDLR) Antibody (CAB20808)
Antibody SKU:CAB20808
Antibody Size:50µL, 100µL
Application:Western blotting, Immunofluorescence
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 761-860 of human LDL Receptor (LDLR) (NP_000518.1).
Application:Western blotting, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-431, HepG2, RAW264.7, Mouse liver, Rat liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 761-860 of human LDL Receptor (LDLR) (NP_000518.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TVEI VTMS HQAL GDVA GRGN EKKP SSVR ALSI VLPI VLLV FLCL GVFL LWKN WRLK NINS INFD NPVY QKTT EDEV HICH NQDG YSYP SRQM VSLE DDVA
Cellular Location:Cell membrane, Cell surface, Early endosome, Endomembrane system, Golgi apparatus, Late endosome, Lysosome, Membrane, Single-pass type I membrane protein, clathrin-coated pit
Calculated MW:75kDa/76kDa/82kDa/90kDa/95kDa
Observed MW:100-160KDa
Synonyms:LDLR, FH, FHC, LDLCQ2
Background:The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.
Immunofluorescence analysis of HepG2 cells using LDL Receptor (LDLR) Rabbit mAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of HepG2 cells using LDL Receptor (LDLR) Rabbit mAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of A-431 cells, using LDL Receptor (LDLR) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of A-431 cells, using LDL Receptor (LDLR) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of HeLa cells using LDL Receptor (LDLR) Rabbit mAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of HeLa cells using LDL Receptor (LDLR) Rabbit mAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using LDL Receptor (LDLR) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Western blot analysis of extracts of various cell lines, using LDL Receptor (LDLR) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

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