The LY6G Polyclonal Antibody (CAB20861) is a high-quality antibody developed for reliable detection and analysis of target proteins. Located in external side of plasma membrane.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse samples.
Product Name:
LY6G Polyclonal Antibody
SKU:
CAB20861
Size:
100μL, 20μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:1000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Gr1, Gr-1, Ly-6G
Positive Sample:
Mouse bone marrow
Cellular Localization:
External Side Of Plasma Membrane.
Calculated MW:
14kDa
Observed MW:
20-40kDa
Located in external side of plasma membrane.
Purification Method
Affinity purification
Gene ID
546644
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse bone marrow, using Ly-6G Rabbit pAb (CAB20861) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of paraffin-embedded mouse spleen using Ly-6G Rabbit pAb (CAB20861) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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