Western blot analysis of extracts of U-87MG cells, using MAN2A2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
The MAN2A2 Polyclonal Antibody (CAB19323) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed for use in various research applications, is specifically designed to detect and analyze MAN2A2 in human samples. Raised in rabbits, it offers high reactivity and specificity in Western blot experiments.MAN2A2, a critical enzyme in glycosylation pathways, plays a pivotal role in the biosynthesis and processing of complex oligosaccharides. Dysregulation of MAN2A2 has been linked to various diseases, including cancer and congenital disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
MAN2A2 Polyclonal Antibody
SKU:
CAB19323
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MANA2X, MAN2A2
Positive Sample:
U-87MG
Calculated MW:
131kDa
Observed MW:
128kDa
Predicted to enable alpha-mannosidase activity. Predicted to be involved in N-glycan processing and protein deglycosylation. Predicted to be integral component of membrane. Predicted to be active in Golgi membrane.
Purification Method
Affinity purification
Gene ID
4122
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from U-87MG cells, using MAN2A2 Rabbit pAb (CAB19323) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
