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Anti-MCM2 Antibody (CAB20699)

SKU:
CAB20699
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
  • Immunofluorescence analysis of U2OS cells using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat spleen using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse lung using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse liver using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Western blot analysis of extracts of various cell lines, using MCM2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 3s.
€179 - €469
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Description

Antibody Name:Anti-MCM2 Antibody (CAB20699)
Antibody SKU:CAB20699
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human MCM2.
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, C2C12
Immunogen:Recombinant protein of human MCM2.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Nucleus
Calculated MW:101kDa
Observed MW:125KDa
Synonyms:MCM2, BM28, CCNL1, CDCL1, D3S3194, DFNA70, MITOTIN, cdc19
Background:The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
Immunofluorescence analysis of U2OS cells using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of U2OS cells using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat spleen using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded rat spleen using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse lung using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded mouse lung using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse liver using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded mouse liver using MCM2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Western blot analysis of extracts of various cell lines, using MCM2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 3s.Western blot analysis of extracts of various cell lines, using MCM2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 3s.

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