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Immunofluorescence analysis of U2OS cells using MonoMethyl-Histone H3-K18 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Key Features

MonoMethyl-Histone H3-K18 Monoclonal Antibody (CAB20680)

Datasheet MSDS
SKU CAB20680
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Host Species Rabbit
Synonyms H3t, H3.4, H3/g, H3FT, H3C16, HIST3H3, MonoMethyl-Histone H3-K18
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

MonoMethyl-Histone H3-K18 Monoclonal Antibody (CAB20680)

The MonoMethyl-Histone H3-K18 Monoclonal Antibody (CAB20680) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically targets the monomethylated form of histone H3 at lysine 18, a post-translational modification associated with gene activation and transcriptional regulation.Raised in rabbits, this antibody is highly specific and sensitive when detecting monomethyl histone H3 (K18) in human samples. It has been validated for use in Western blot applications, allowing for accurate analysis of histone modifications in different cell types and biological contexts.Histone modifications, such as monomethylation of histone H3 at K18, play a key role in gene expression regulation and epigenetic control.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ChIP, ELISA, DB, CUT&Tag applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.

Product Name:MonoMethyl-Histone H3-K18 Monoclonal Antibody
SKU:CAB20680
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Clone Number:ARC2621
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:APRK Q
Tested Applications:WB IHC-P IF/ICC IP ChIP ELISA DB CUT&Tag
Recommended Dilution:
WB1:500 - 1:1000
DB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
IP2μg-6μg antibody for 400μg-600μg extracts of whole cells
ChIP5μg antibody for 5μg-10μg of Chromatin
CUT&Tag10⁵ cells /1 μg
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:H3t, H3.4, H3/g, H3FT, H3C16, HIST3H3, MonoMethyl-Histone H3-K18
Positive Sample:HeLa nuclear extract, NIH/3T3 nuclear extract, C6 nuclear extract
Cellular Localization:Chromosome, Nucleus.
Calculated MW:15kDa
Observed MW:17kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Purification MethodAffinity purification
Gene ID8290 8350
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using MonoMethyl-Histone H3-K18 Rabbit mAb (CAB20680) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageDot-blot analysis of all sorts of peptides using MonoMethyl-Histone H3-K18 antibody (CAB20680) at 1:1000 dilution.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse lung using MonoMethyl-Histone H3-K18 Rabbit mAb (CAB20680) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain using MonoMethyl-Histone H3-K18 Rabbit mAb (CAB20680) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H3-K18 Rabbit mAb (CAB20680) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunoprecipitation analysis of 600 μg extracts of 293F cells using 5 μg MonoMethyl-Histone H3-K18 antibody (CAB20680). Western blot was performed from the immunoprecipitate using MonoMethyl-Histone H3-K18 antibody (CAB20680) at a dilution of 1:1000.
Product ImageChromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K18 antibody (CAB20680) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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