Anti-Mouse CD137L (4-1BBL) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD137L (4-1BBL) In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD137L protein, a crucial regulatory component in the immune response, making it ideal for research in immunology and related fields. With a rat IgG2a kappa isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), making it perfect for ELISA, flow cytometry, immunohistochemistry, and other related assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. CD137L, also known as 4-1BB ligand, is a protein involved in the regulation of T-cell activation and survival. It plays a significant role in the immune system by providing crucial signals for the proliferation and differentiation of immune cells, particularly T-cells, through its interaction with CD137 (4-1BB) on the surface of these cells. This makes it a vital tool for exploring immune response mechanisms and therapeutic strategies in immunology.
Product Name:
Anti-Mouse CD137L (4-1BBL) In Vivo Antibody - Low Endotoxin
Product Code:
IVMB0380
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
TKS-1
Protein:
4-1BBL
Product Type:
Monoclonal Antibody
Synonyms:
4-1BBL, CD137L, TNFSF9, 4-1BB Ligand, TKS-1
Isotype:
IgG2a k
Reactivity:
Mouse
Applications:
FC, In Vivo
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
<1.0 EU/µg as determined by the LAL method
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
FC, In Vivo
Reactivity:
Mouse
Host Species:
Rat
Specificity:
TKS-1 activity is directed against 4-1BBL (CD137L).
Antigen Distribution:
4-1BBL is expressed by activated B cells, macrophages, and dendritic cells.
Immunogen:
BALB/c mouse B lymphoma line 2PK-3
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
<1.0 EU/µg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
4-1BBL (CD137L) and 4-1BB (CD137) are a receptor ligand pair belonging to the tumor necrosis factor (TNF) receptor/TNF superfamily1,2. 4-1BBL is a type II transmembrane protein expressed by splenic B and T cells, macrophages, and dendritic cells1-5. Members of the TNF-TNF receptor superfamily play critical roles in regulating cellular activation, differentiation, and apoptosis6 and the 4-1BBL-4-1BB interaction is important in cellular immune responses5. 4-1BBL-4-1BB interaction provides a co-stimulatory signal to T cells, resulting in increased proliferation and cytokine production5.
A TKS-1-producing hybridoma was generated by immunizing a SD rat with 4-1BBL-transfected NRK cells (rat IgG2a,κ)5. Splenocytes were fused with P3U1 cells and hypoxanthine–aminopterin–thymidine selection was performed. The monoclonal antibody was identified by its strong reactivity with 4-1BBL-transfected L5178Y cells, cloned by limiting diffusion, purified from ascites, and found to bind to 4-1BBL/NRK and 4-1BBL/P815 cells5.
Pre-incubation with TKS-1 blocks 4-1BB–Ig binding to 4-1BBL/L5178Y cells, indicating TKS-1 is specific to mouse 4-1BBL and can interrupt the interaction between 4-1BBL and 4-1BB5. Additionally, TKS-1 can block anti-CD3-stimulated T cell proliferation co-stimulated by 4-1BBL /P815 cells. The monoclonal antibodies 19H3 and TKS-1 bind to different sites on murine 4-1BBL, with TKS-1 binding to a site that overlaps with the receptor binding site7. Additionally, TKS-1 and 4-1BB bind to a similar site, making TKS-1 especially useful for blocking studies.
