Description
Anti-Mouse CD172a In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD172a In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD172a protein, which is pivotal in cellular signaling and immune response, making it indispensable for research in immunology and related fields. With a rat IgG2b isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), suitable for various assays such as ELISA, flow cytometry, and functional studies. Available in diverse sizes, it is formulated in phosphate-buffered saline to ensure stability and efficacy.
Elevate your research with this reliable and versatile antibody. CD172a, also known as SIRP-alpha (Signal Regulatory Protein Alpha), is a glycoprotein expressed on the surface of myeloid cells such as monocytes, macrophages, granulocytes, and dendritic cells. It plays a crucial role in the negative regulation of receptor tyrosine kinase-coupled signaling processes which is vital for immune cell signaling and response regulation.
| Product Name: | Anti-Mouse CD172a In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0029 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | P84 |
| Protein: | CD172a |
| Product Type: | Monoclonal Antibody |
| Synonyms: | SIRPα, SHPS-1, BIT, P84, PTPNS1, CD172 antigen-like family member A |
| Isotype: | Rat IgG1 κ |
| Reactivity: | Mouse |
| Immunogen: | Mouse brain membrane protein |
| Applications: | B, CyTOF®, FC, IHC FF, IP |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CyTOF®, FC, IHC FF, IP |
| Recommended Usage: | FC The suggested concentration for this P84 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. CyTOF® |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone P84 recognizes an epitope on mouse CD172a. |
| Antigen Distribution: | CD172a is expressed on monocytes, macrophages, dendritic cells, and neuronal cells. |
| Immunogen: | Mouse brain membrane protein |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD172a antibody, clone P84, recognizes CD172a, also known as single regulatory protein α (SIRPα) (signal regulatory protein alpha) or Src homology 2 domain-containing phosphatase substrate-1 (SHP-1), a type I transmembrane glycoprotein with three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibition motifs (ITIMs)1. SIRPα is expressed predominantly in myeloid cells2 - including monocytes, macrophages, and dendritic cells (DCs) - and neuronal cells3. The extracellular ligand for SIRPα, CD47 (or integrin-associated protein [IAP])4, is expressed in most cell types5. In macrophages, ligation of SIRPα by CD47 inhibits macrophage phagocytosis of self cells6,7. SIRPα also negatively regulates DC-mediated T cell activation and DC maturation8-10. CD47 is also upregulated on tumor cells, inhibiting the phagocytosis of tumor cells by macrophages11. Therapeutics targeting the CD47-SIRPα interaction, including antibodies and fusion proteins, are currently under preclinical and clinical study for various malignancies as a monotherapy or in combination with other therapeutics12.
| Technical Datasheet: | View |
| Protein: | CD172a |
| Function: | Negative regulation of several biological processes |
| Ligand/Receptor: | CD47, SP-A, SP-D |
| Research Area: | Cell Adhesion, Innate Immunity, Neuroscience |
