Anti-Mouse CD24 In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse CD24 In Vivo Antibody – Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD24 protein, which is critically involved in various immune processes, making it ideal for research in immunology and related fields. With a rat IgG2b isotype, it ensures high purity and ultra-low endotoxin levels (<0.002 EU/μg), perfect for applications such as ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. CD24, also known as Heat Stable Antigen (HSA), is a cell surface protein expressed on a variety of cell types, including hematopoietic and neural cells. It plays a significant role in cell adhesion and signal transduction, particularly in the immune system. The interaction of CD24 with its ligands is crucial for the regulation of immune responses and in the development and function of immune cells.
Product Name:
Anti-Mouse CD24 In Vivo Antibody - Ultra Low Endotoxin
Product Code:
IVMB0291
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
M1/69
Protein:
CD24
Product Type:
Monoclonal Antibody
Synonyms:
Heat Stable Antigen, Nectadrin, Ly-52
Isotype:
IgG2b k
Reactivity:
Mouse
Applications:
Comp Inhib, ELISA Indirect, FACS, FC, IF, IF Microscopy, IHC, In Vivo, IP, WB
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Purity:
≥98% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
Comp Inhib, ELISA Indirect, FACS, FC, IF, IF Microscopy, IHC, In Vivo, IP, WB
Reactivity:
Mouse
Host Species:
Rat
Specificity:
Anti-CD24 antibody (clone M1/69) activity is directed against mouse CD24, also known as Heat Stable Antigen (HSA) or Ly-52.
Antigen Distribution:
CD24 is expressed on B cells, T cells, neutrophils, eosinophils, macrophages, neural cells, ganglion cells, keratinocytes, muscle cells, pancreas cells, lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells and is overexpressed in many cancers. Expression varies during T and B cell differentiation. Peripheral T cells are mainly negative.
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥98% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Mouse CD24 is a small 27 amino acid sialoglycoprotein that is anchored to plasma membranes via a glycosyl-phosphatidylinositol linker1. CD24 is widely distributed and plays a role in many diverse functions including adaptive immunity, inflammation, autoimmunity, and cancer1,2. CD24 modulates growth and differentiation signals to granulocytes and B cells, is required for homeostatic cell renewal, binds to P-selectin on activated endothelial cells, plays a role in cell adhesion3, lymphocyte proliferation for homeostatic purposes4, lymphocyte costimulation via CD28-independent pathways5 as well as a crucial role in cell selection and maturation during hematopoiesis.
CD24 tends to be expressed more abundantly in progenitor cells and metabolically active cells relative to terminally differentiated cells2. For example, CD24 is expressed on B-cell progenitors and mature resting B cells, but not terminally differentiated plasma cells. Similarly, CD24 is abundantly expressed on immature T cells and activated T cells but weakly expressed on peripheral T cells. As such, CD24 is used as a marker for the differentiation of hematopoietic and neuronal cells as well as tumor stem cells.
In humans, CD24 is overexpressed in various malignancies and its downregulation reduces cell tumorgenicity6. When CD24 is expressed early during carcinogenesis and blocked with monoclonal antibodies or small interfering RNA, tumor growth in xenograft mouse models is reduced.
M1/69 was generated by fusing the spleen cells of a DA rat immunized with B10 mouse spleen cells enriched for T cells with cells from a nonsecreting mouse myeloma line (NSI)7.
Protein:
CD24
Ligand/Receptor:
P-selectin, CD24
Research Area:
Cell Biology, Immunology
Pathogen Testing:
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
