Anti-Mouse CD4 [GK1.5] In Vivo Antibody

Product Name:	Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0061
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	GK1.5
Protein:	CD4
Product Type:	Monoclonal Antibody
Synonyms:	L3T4, T4
Isotype:	Rat IgG2b κ
Reactivity:	Mouse
Immunogen:	Mouse CTL clone V4
Applications:	B, Costim, CyTOF®, Depletion, FA, FC, IHC, IP
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0060	Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Low Endotoxin	Low
IVMB0061	Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	B, Costim, CyTOF®, Depletion, FA, FC, IHC, IP
Recommended Usage:	CyTOF®FC The suggested concentration for this GK1.5 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 10⁶ in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern.
Antigen Distribution:	The CD4/L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Immunogen:	Mouse CTL clone V4
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.6

Technical Datasheet:	View
Protein:	CD4
Ligand/Receptor:	MHC class II molecule
Research Area:	Immunology

Rat IgG2b Isotype Control
Clone	1-2
Isotype	Rat IgG2b kappa
Endotoxin Level	Ultra Low Endotoxin

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