Anti-Mouse CD54 [YN1/1.7.4] In Vivo Antibody

Product Name:	Anti-Mouse CD54 [YN1/1.7.4] In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0062
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	YN1/1.7.4
Protein:	CD54
Product Type:	Monoclonal Antibody
Synonyms:	Ly-47, ICAM-1
Isotype:	Rat IgG2b κ
Reactivity:	Mouse
Immunogen:	Mouse NS-1 cells
Applications:	CyTOF®, FA, FC, IHC FF, IP, PhenoCycler®, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0062	Anti-Mouse CD54 [YN1/1.7.4] In Vivo Antibody - Low Endotoxin	Low
IVMB0063	Anti-Mouse CD54 [YN1/1.7.4] In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	CODEX®, CyTOF®, FA, FC, IHC FF, IP, WB
Recommended Usage:	FC The suggested concentration for this YN1/1.7.4 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 10⁶ in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this YN1/1.7.4 antibody for use in western blotting is 1-10 µg/ml.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone YN1/1.7.4 recognizes an epitope on mouse CD54.
Antigen Distribution:	CD54 is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes.
Immunogen:	Mouse NS-1 cells
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.

Technical Datasheet:	View
Protein:	CD54
Function:	Immune reaction, inflammation, adhesion
Ligand/Receptor:	CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Research Area:	Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cell

Rat IgG2b Isotype Control
Clone	1-2
Isotype	Rat IgG2b
Endotoxin Level	Low Endotoxin

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