Anti-Mouse IL-4 In Vivo Antibody

Product Name:	Anti-Mouse IL-4 In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0086
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	11B11
Protein:	IL-4
Product Type:	Monoclonal Antibody
Synonyms:	Interleukin-4, BCGF, BCDF, B Cell Stimulating Factor, BSF-1, IaIF, HCGF, MCGF2, MFF, TCGF-2
Isotype:	Rat IgG1 κ
Reactivity:	Mouse
Immunogen:	Partially Purified Native Mouse IL-4
Applications:	CyTOF®, ELISA Cap, ELISPOT, FA, ICC, IHC, IP, N
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Applications:	CyTOF®, ELISA Cap, ELISPOT, FA, ICC, IHC, IP, N
Recommended Usage:	ELISA This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 µg/ml. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone 11B11 recognizes an epitope on mouse IL-4.
Antigen Distribution:	IL-4 is secreted by Th2 cells, NKT cells, mast cells, eosinophils, and basophils.
Immunogen:	Partially Purified Native Mouse IL-4
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

IL-4 antibody, 11B11, recognizes mouse IL-4, also known as B-cell differentiation factor (BCDF) and B-cell stimulatory factor (BSF1). IL-4 is a pleiotropic 20 kDa cytokine secreted by T helper 2 cells (Th2) cells, natural killer T (NKT) cells, mast cells, eosinophils, and basophils1,2. IL-4 signaling through the IL-4 receptor alpha chain (IL-4Ra) induces the differentiation of naive T cells to Th2 cells3,4 and stimulates B cell differentiation, proliferation, and class switching to IgG1 and IgE isotypes5. In addition, IL-4 is implicated in normal wound healing6,7 and airway inflammation in patients with asthma8.

Technical Datasheet:	View
Protein:	IL-4
Research Area:	Immunology

Rat IgG1 Isotype Control
Clone	GL113
Isotype	Rat IgG1
Endotoxin Level	Low Endotoxin

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