Western blot analysis of extracts of various cell lines, using PIF4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
The PIF4 Polyclonal Antibody (CAB20725) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting PIF4 in plant samples, particularly in Western blot applications. By targeting the PIF4 protein, this antibody allows for the accurate identification and analysis of PIF4 expression levels in different plant species and tissues.PIF4, also known as phytochrome-interacting factor 4, plays a crucial role in mediating light signaling and regulating various physiological processes in plants, such as seed germination, seedling growth, and flowering time.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Arabidopsis thaliana samples.
Product Name:
PIF4 Polyclonal Antibody
SKU:
CAB20725
Size:
20μL, 100μL
Reactivity:
Arabidopsis thaliana
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isolated as a semidominant mutation defective in red -light responses. Encodes a nuclear localized bHLH protein that interacts with active PhyB protein. Negatively regulates phyB mediated red light responses. Involved in shade avoidance response. Protein abundance is negatively regulated by PhyB.
Purification Method
Affinity purification
Gene ID
818903
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using PIF4 Rabbit pAb (CAB20725) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
