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Anti-PKM1-specific Antibody (CAB18800)

SKU:
CAB18800
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
PK3
TCB
p58
OIP3
PKM2
CTHBP
THBP1
Research Area:
Metabolism
$167 - $503
Frequently bought together:

Description

Product Name:PKM1-specific Rabbit pAb
Product Code:CAB18800
Size:20uL, 50uL, 100uL
Synonyms:PK3, TCB, p58, OIP3, PKM2, CTHBP, THBP1,
Applications:WB, IHC, IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human PKM1-specific.
Applications:WB, IHC, IF
Recommended Dilutions:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse skeletal muscle, Mouse heart, Rat skeletal muscle
Immunogen:Recombinant protein of human PKM1-specific.
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5315
Uniprot:P14618
Cellular Location:Cytoplasm, Nucleus
Calculated MW:56kDa/57kDa/58kDa
Observed MW:58kDa
UniProt Protein Function:PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.1.40; Carbohydrate Metabolism - pyruvate; Mitochondrial; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: extracellular matrix; mitochondrion; cytoplasm; plasma membrane; nucleus; cytosol; vesicle; cilium

Molecular Function:potassium ion binding; protein binding; magnesium ion binding; pyruvate kinase activity; ATP binding

Biological Process: glycolysis; carbohydrate metabolic process; programmed cell death; glucose metabolic process; pathogenesis; phosphorylation

NCBI Summary:This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
UniProt Code:P14618
NCBI GenInfo Identifier:20178296
NCBI Gene ID:5315
NCBI Accession:P14618.4
UniProt Secondary Accession:P14618,P14786, Q53GK4, Q96E76, Q9BWB5, Q9UCV6, Q9UPF2 A6NFK3, B2R5N8, B3KRY0, B4DFX8, B4DUU6,
UniProt Related Accession:P14618
Molecular Weight:
NCBI Full Name:Pyruvate kinase PKM
NCBI Synonym Full Names:pyruvate kinase, muscle
NCBI Official Symbol:PKM  
NCBI Official Synonym Symbols:PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30  
NCBI Protein Information:pyruvate kinase PKM; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; epididymis secretory protein Li 30; cytosolic thyroid hormone-binding protein; thyroid hormone-binding protein, cytosolic
UniProt Protein Name:Pyruvate kinase PKM
UniProt Synonym Protein Names:Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58
Protein Family:Pyruvate kinase
UniProt Gene Name:PKM  
UniProt Entry Name:KPYM_HUMAN

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