Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
The PPM1E Monoclonal Antibody (CAB9374) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is recommended for use in Western blot applications and has been validated for its ability to detect PPM1E in human samples.PPM1E is a phosphatase enzyme that plays a pivotal role in cellular processes such as cell cycle regulation, DNA repair, and apoptosis. Dysregulation of PPM1E has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. Therefore, studying the expression and function of PPM1E is essential for understanding its role in disease progression and identifying potential therapeutic targets.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PPM1E Monoclonal Antibody
SKU:
CAB9374
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2763
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
POPX1, PP2CH, caMKN, CAMKPN, CaMKP-N, PPM1E
Positive Sample:
293T, SH-SY5Y
Cellular Localization:
Mitochondrion, Nucleolus, Nucleoplasm, Nucleus.
Calculated MW:
84kDa
Observed MW:
85kDa
This gene encodes a member of the PPM family of serine/threonine-protein phosphatases. The encoded protein is localized to the nucleus and dephosphorylates and inactivates multiple substrates including serine/threonine-protein kinase PAK 1, 5'-AMP-activated protein kinase (AMPK) and the multifunctional calcium/calmodulin-dependent protein kinases. Alternatively spliced transcript variants have been observed for this gene.
Purification Method
Affinity purification
Gene ID
22843
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PPM1E Rabbit mAb (CAB9374) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180 s.
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