Western blot analysis of extracts of various cell lines, using Pyrophosphatase 1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
The Pyrophosphatase 1 Monoclonal Antibody (CAB20942) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications. By specifically binding to pyrophosphatase-1, this antibody enables accurate detection and analysis of the enzyme in various cell types, making it ideal for investigations in biochemistry and molecular biology.Pyrophosphatase-1 plays a crucial role in cellular metabolism by catalyzing the hydrolysis of inorganic pyrophosphate, a byproduct of many biochemical reactions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Pyrophosphatase 1 Monoclonal Antibody
SKU:
CAB20942
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2928
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
MCF7, Mouse large intestine, Mouse liver, Rat liver
Cellular Localization:
Cytoplasm.
Calculated MW:
33kDa
Observed MW:
33kDa
The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme.
Purification Method
Affinity purification
Gene ID
5464
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Pyrophosphatase 1 Rabbit mAb (CAB20942) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
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