The RUNX2 Monoclonal Antibody (CAB11753) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has been validated for use in various applications, including Western blot and immunohistochemistry. By specifically binding to the Runx2 protein, this antibody enables accurate detection and analysis in a wide range of cell types and tissues.Runx2, a transcription factor essential for bone development, has also been implicated in various diseases, including osteoporosis and certain types of cancer.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
RUNX2 Monoclonal Antibody
SKU:
CAB11753
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC0362
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing.
Purification Method
Affinity purification
Gene ID
860
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RUNX2 Rabbit mAb (CAB11753) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using RUNX2 Rabbit mAb (CAB11753) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
