Description
SARS-CoV-2 NSP3 Antibody (CAB20236)
The SARS-CoV-2 NSP3 Antibody (CAB20236) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced and validated for use in various applications, including ELISA, Western blot, and immunohistochemistry, is highly specific to the NSP3 protein and is suitable for studying viral pathogenesis and host immune response to SARS-CoV-2 infection.NSP3 is a key protein of SARS-CoV-2 that plays a crucial role in viral replication and modulating host immune responses, making it a promising target for drug development and vaccine design.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against SARS-CoV-2 samples.
| Product Name: | SARS-CoV-2 NSP3 Antibody |
| SKU: | CAB20236 |
| Size: | 20μL, 100μL |
| Reactivity: | SARS-CoV-2 |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | MAPT KVTF GDDT VIEV QGYK SVNI TFEL DERI DKVL NEKC SAYT VELG TEVN EFAC VVAD AVIK TLQP VSEL LTPL GIDL DEWS MATY YLFD ESGE FKLA SHMY CSFY PPDE DEEE GDCE EEEF EPST QYEY GTED DYQG KPLE FGAT SAAL QPEE EQEE DWLD DDSQ QTVG QQDG SEDN QTTT IQTI VEVQ PQLE MELT | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Positive Sample: | 293T |
| Calculated MW: | 794kDa |
| Observed MW: | 33kDa |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.
| Purification Method | Affinity purification |
| Gene ID | 43740578 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of extracts of normal 293T cells 293T transfected with NSP3 Protein, using SARS-CoV-2 NSP3 Rabbit pAb (CAB20236) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. |
