The SARS-CoV Spike Antibody (CAB20605) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody is specifically designed to target the spike protein, aiding in the detection and analysis of viral particles in various samples. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in applications such as Western blotting. Its ability to bind to the SARS-CoV spike protein makes it an essential tool for studying the structure and function of this key viral component, providing insights that can inform the development of diagnostics, treatments, and vaccines for SARS-CoV infections.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against SARS-CoV samples.
Product Name:
SARS-CoV Spike Antibody
SKU:
CAB20605
Size:
20μL, 100μL
Reactivity:
SARS-CoV
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
E2, SARS-CoV Spike
Positive Sample:
293T
Calculated MW:
139kDa
Observed MW:
200kDa
The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Purification Method
Affinity purification
Gene ID
1489668
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SARS-CoV Spike Rabbit pAb (CAB20605) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
