Anti-SF3B1 Antibody (CAB9737)

Product Name:	SF3B1 Rabbit mAb
Product Code:	CAB9737
Size:	20uL, 50uL, 100uL
Synonyms:	Hsh155, MDS, PRP10, PRPF10, SAP155, SF3b155
Applications:	WB, IHC, IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human SF3B1

Applications:	WB, IHC, IF
Recommended Dilutions:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, NIH/3T3, Jurkat, Mouse brain

Immunogen:	A synthesized peptide derived from human SF3B1
Purification Method:	Affinity purification
Storage:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	23451
Uniprot:	O75533
Calculated MW:	155kDa
Observed MW:	146KDa

UniProt Protein Function:	SF3B1: subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (bps) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Phosphorylated during the splicing catalytic steps.
UniProt Protein Details:	Protein type:RNA-binding; Spliceosome; RNA splicing Chromosomal Location of Human Ortholog: 2q33.1 Cellular Component: nucleoplasm; nucleus; snRNP U2; U12-dependent spliceosome Molecular Function:mRNA binding; protein binding Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of gene expression, epigenetic; spliceosome assembly Disease: Myelodysplastic Syndrome
NCBI Summary:	This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	O75533
NCBI GenInfo Identifier:	269849656
NCBI Gene ID:	23451
NCBI Accession:	O75533.3
UniProt Secondary Accession:	O75533,E9PCH3,
UniProt Related Accession:	O75533
Molecular Weight:	16,020 Da
NCBI Full Name:	Splicing factor 3B subunit 1
NCBI Synonym Full Names:	splicing factor 3b subunit 1
NCBI Official Symbol:	SF3B1
NCBI Official Synonym Symbols:	MDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155
NCBI Protein Information:	splicing factor 3B subunit 1
UniProt Protein Name:	Splicing factor 3B subunit 1
UniProt Synonym Protein Names:	Pre-mRNA-splicing factor SF3b 155 kDa subunit; SF3b155; Spliceosome-associated protein 155; SAP 155
Protein Family:	Splicing factor
UniProt Gene Name:	SF3B1
UniProt Entry Name:	SF3B1_HUMAN