Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
The Spermine synthase Monoclonal Antibody (CAB9380) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It binds specifically to the spermine synthase protein, allowing for accurate detection and analysis in various cell types, making it ideal for studies in biochemistry and cancer research.Spermine synthase plays a crucial role in polyamine metabolism, which is essential for cell proliferation and survival.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Spermine synthase Monoclonal Antibody
SKU:
CAB9380
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2747
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SRS, SpS, MRSR, SPMSY, MRXSSR, Spermine synthase
Positive Sample:
293T, DU 145, SH-SY5Y, Mouse brain, Rat brain
Cellular Localization:
Cytosol, Extracellular Exosome.
Calculated MW:
41kDa
Observed MW:
41kDa
This gene encodes a protein belonging to the spermidine/spermin synthase family and catalyzes the production of spermine from spermidine. Pseudogenes of this gene are located on chromosomes 1, 5, 6 and X. Mutations in this gene cause an X-linked intellectual disability called Snyder-Robinson Syndrome (SRS). Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6611
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using (CAB9380) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
Western blot analysis of various lysates using Spermine synthase Rabbit mAb (CAB9380) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
![Anti-Spermine Synthase [R05-5J5] Monoclonal Antibody (AGMB02354)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/273643/678791/anti-spermine-synthase-r05-5j5-monoclonal-antibody-agmb02354__91155.1773036867.jpg?c=2 "Anti-Spermine Synthase [R05-5J5] Monoclonal Antibody (AGMB02354)")
![Anti-Thymidylate Synthase [ZN227] Monoclonal Antibody (AGMB06270)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/277551/680307/anti-thymidylate-synthase-zn227-monoclonal-antibody-agmb06270__68513.1773041664.jpg?c=2 "Anti-Thymidylate Synthase [ZN227] Monoclonal Antibody (AGMB06270)")
