The THRAP3 Antibody (CAB9396) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through immunization in rabbits, is particularly reactive with human samples and has been validated for use in various applications, including Western blotting.THRAP3, also known as thyroid hormone receptor-associated protein 3, is a key player in transcriptional regulation and RNA splicing processes, making it a significant target in studies related to gene expression and RNA metabolism.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
THRAP3 Antibody
SKU:
CAB9396
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BCLAF2, TRAP150, THRAP3
Positive Sample:
HeLa
Cellular Localization:
Nucleus, Nucleus Speckle, Nucleoplasm.
Calculated MW:
109kDa
Observed MW:
150kDa
Enables phosphoprotein binding activity; thyroid hormone receptor binding activity; and transcription coactivator activity. Involved in nuclear-transcribed mRNA catabolic process; positive regulation of circadian rhythm; and regulation of RNA metabolic process. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Located in nuclear speck. Part of mediator complex. Colocalizes with exon-exon junction complex.
Purification Method
Affinity purification
Gene ID
9967
RRID
AB_2772597
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using THRAP3 Rabbit pAb (CAB9396) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human brain tissue using THRAP3 Rabbit pAb (CAB9396) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using THRAP3 Rabbit pAb (CAB9396) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
