Wilms Tumor Protein Rabbit Monoclonal Antibody (CAB2446)
- SKU:
- CAB2446
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- WT1
- AWT1
- EWS-WT1
- GUD
- NPHS4
- WAGR
- WIT-2
- WT33
- Wilms tumor 1
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Product Name: | Wilms Tumor Protein Rabbit Monoclonal Antibody |
| SKU: | CAB2446 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human WT1 (P19544). |
| Sequence: | PPPP PPPP PHSF IKQE PSWG GAEP HEEQ CLSA FTVH FSGQ FTGT AGAC RYGP FGPP PPSQ ASSG QARM FPNA PYLP SCLE SQPA IRNQ GYST VTFD GTPS YGHT PSHH AAQF PNHS FKHE DPMG QQGS LGEQ QYSV PPPV YGCH TPTD SCTG SQAL LLRT PYSS DNLY QMTS QLEC MTWN QMNL GATL KGV |
| Tested Applications: | WB IHC-P ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 |
| Synonyms: | GUD; AWT1; WAGR; WT-1; WT33; NPHS4; WIT-2; WT1 |
| Positive Sample: | OVCAR3,K-562,Mouse testis,Rat testis |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Nucleus, Nucleus speckle, nucleolus, nucleoplasm. |
| Calculated MW: | 49kDa |
| Observed MW: | 51-60kDa |
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
| Purification Method: | Affinity purification |
| Gene ID: | 7490 |
| Clone Number: | ARC2610 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using WT1 Rabbit mAb (CAB2446) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s. |
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