The Annexin A5/Annexin V Antibody (CAB13945) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the ANXA5 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for studies in cell biology, apoptosis, and cancer research.ANXA5, also known as annexin A5, is a calcium-dependent phospholipid-binding protein that plays a crucial role in the regulation of cell death processes.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Annexin A5/Annexin V Antibody
SKU:
CAB13945
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PP4, ANX5, ENX2, CPB-I, RPRGL3, HEL-S-7, VAC-alph, Annexin A5/Annexin V
Positive Sample:
HepG2, HeLa
Cellular Localization:
Cytoplasm, Cytosol, External Side Of Plasma Membrane, Extracellular Exosome, Extracellular Region, Focal Adhesion.
Calculated MW:
36kDa
Observed MW:
36kDa
The Annexin 5 gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. Polymorphisms in this gene have been implicated in various obstetric complications.
Purification Method
Affinity purification
Gene ID
308
RRID
AB_2760798
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Annexin A5/Annexin V Rabbit pAb (CAB13945) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human colon using Annexin A5/Annexin V Rabbit pAb (CAB13945) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Annexin A5/Annexin V Rabbit pAb (CAB13945) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
