Anti-ANXA4 Antibody (CAB13466)

Antibody Name:	Anti-ANXA4 Antibody
Antibody SKU:	CAB13466
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 112-321 of human ANXA4 (NP_001144.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 112-321 of human ANXA4 (NP_001144.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IEIL ASRT PEEI RRIS QTYQ QQYG RSLE DDIR SDTS FMFQ RVLV SLSA GGRD EGNY LDDA LVRQ DAQD LYEA GEKK WGTD EVKF LTVL CSRN RNHL LHVF DEYK RISQ KDIE QSIK SETS GSFE DALL AIVK CMRN KSAY FAEK LYKS MKGL GTDD NTLI RVMV SRAE IDML DIRA HFKR LYGK SLYS FIKG DTSG DYRK VLLV LCGG DD
Gene ID:	307
Uniprot:	P09525
Cellular Location:
Calculated MW:	27kDa/35kDa
Observed MW:	36kDa

Synonyms:

ANXA4, ANX4, HEL-S-274, P32.5, PAP-II, PIG28, PP4-X, ZAP36

Background:

Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ANXA4: a calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.
UniProt Protein Details:	Protein type:Lipid-binding; Calcium-binding Chromosomal Location of Human Ortholog: 2p13 Cellular Component: nuclear membrane; cell surface; perinuclear region of cytoplasm; cytoplasm; plasma membrane; vesicle membrane; nucleus Molecular Function:phospholipase inhibitor activity; identical protein binding; NF-kappaB binding; calcium-dependent phospholipid binding; calcium ion binding; calcium-dependent protein binding Biological Process: regulation of transcription from RNA polymerase II promoter; epithelial cell differentiation; inhibition of NF-kappaB transcription factor; negative regulation of catalytic activity; signal transduction; negative regulation of apoptosis
NCBI Summary:	Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. [provided by RefSeq, Jul 2008]
UniProt Code:	P09525
NCBI GenInfo Identifier:	1703319
NCBI Gene ID:	307
NCBI Accession:	P09525.4
UniProt Secondary Accession:	P09525,Q96F33, Q9BWK1,
UniProt Related Accession:	P09525
Molecular Weight:	35,883 Da
NCBI Full Name:	Annexin A4
NCBI Synonym Full Names:	annexin A4
NCBI Official Symbol:	ANXA4
NCBI Official Synonym Symbols:	ANX4; PIG28; ZAP36; HEL-S-274
NCBI Protein Information:	annexin A4; P32.5; PP4-X; PAP-II; annexin-4; protein II; endonexin I; lipocortin IV; chromobindin-4; 35-beta calcimedin; proliferation-inducing gene 28; proliferation-inducing protein 28; placental anticoagulant protein II; epididymis secretory protein Li 274; carbohydrate-binding protein p33/p41; annexin IV (placental anticoagulant protein II)
UniProt Protein Name:	Annexin A4
UniProt Synonym Protein Names:	35-beta calcimedin; Annexin IV; Annexin-4; Carbohydrate-binding protein p33/p41; Chromobindin-4; Endonexin I; Lipocortin IV; P32.5; PP4-X; Placental anticoagulant protein II; PAP-II; Protein II
Protein Family:	Annexin
UniProt Gene Name:	ANXA4
UniProt Entry Name:	ANXA4_HUMAN

	Western blot analysis of extracts of various cell lines, using ANXA4 antibody (CAB13466) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of HeLa cells using ANXA4 antibody (CAB13466). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-ANXA4 Antibody (CAB6280)	Human ANXA4 ELISA Kit
Anti-Annexin IV Antibody (CAB9203)	Human Annexin A4 (ANXA4) ELISA Kit
ANXA4 Antibody (PACO07754)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)