| UniProt Protein Function: | APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing.Protein type: ApoptosisChromosomal Location of Human Ortholog: 12q23.1Cellular Component: apoptosome; cytosolMolecular Function: nucleotide binding; protein bindingBiological Process: apoptosis; caspase activation; caspase activation via cytochrome c; nervous system development; positive regulation of apoptosis; regulation of apoptosis |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O14727-6 |
| NCBI GenInfo Identifier: | 20141188 |
| NCBI Gene ID: | 317 |
| NCBI Accession: | O14727.2 |
| UniProt Secondary Accession: | O14727-6,O43297, Q7Z438, Q9BXZ6, Q9UBZ5, Q9UGN8, Q9UGN9 Q9UGP0, Q9UJ58, Q9UJ59 |
| UniProt Related Accession: | O14727-6,O14727 |
| Molecular Weight: | 37,976 Da |
| NCBI Full Name: | Apoptotic protease-activating factor 1 |
| NCBI Synonym Full Names: | apoptotic peptidase activating factor 1 |
| NCBI Official Symbol: | APAF1Â Â |
| NCBI Official Synonym Symbols: | CED4; APAF-1Â Â |
| NCBI Protein Information: | apoptotic protease-activating factor 1 |
| UniProt Protein Name: | Apoptotic protease-activating factor 1 |
| UniProt Synonym Protein Names: | |
| Protein Family: | Apoptotic protease-activating factor |
| UniProt Gene Name: | APAF1Â Â |
| UniProt Entry Name: | |
