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APC Anti-Mouse CD34 Antibody [RAM34] (AGEL3007)

Datasheet MSDS
SKU AGEL3007
Product Type Antibody
Antibody Type Monoclonal Antibody
Applications FCM
Reactivity Mouse
Host Species Rat
Isotype Rat IgG2a, κ
Clone RAM34
Conjugation APC
$143 - $618

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Description

system_update_altDatasheetsystem_update_altMSDS

APC Anti-Mouse CD34 Antibody [RAM34]

The Ram34 Monoclonal Antibody (AGEL3007) is a powerful tool for researchers studying mouse CD34, a pivotal cell surface marker involved in hematopoietic stem cell regulation and vascular endothelial cell differentiation. This antibody, specifically targeting mouse CD34, is generated from a hybridoma cell line and demonstrates high specificity and sensitivity in immunohistochemistry and flow cytometry applications.CD34 is a key marker for identifying hematopoietic stem and progenitor cells, as well as endothelial progenitor cells, in mouse models. By binding to CD34, the Ram34 Monoclonal Antibody enables precise detection and characterization of these cell populations in various tissues and experimental settings.

Its reliable performance makes it an invaluable tool for investigating stem cell biology, vascular development, and regenerative medicine in preclinical research.With its ability to accurately target mouse CD34, the Ram34 Monoclonal Antibody opens up new avenues for understanding the role of this marker in physiological processes and disease pathogenesis. Researchers studying hematopoiesis, angiogenesis, and stem cell transplantation will benefit from the robust capabilities of this antibody in their investigations. Unleash the potential of your mouse CD34 research with the Ram34 Monoclonal Antibody.

Product Title:APC Anti-Mouse CD34 Antibody [RAM34]
SKU:AGEL3007
Size:25µg, 100µg
Clone No:RAM34
Synonyms:Hematopoietic progenitor cell antigen CD34;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG2a, κ
Isotype Control:APC Rat IgG2a, κ Isotype Control[2A3]
Form:Liquid
Conjugation:APC
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:Spectrum Image
Swissprot:Q64314
Gene ID:12490
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:The RAM34 antibody reacts with the CD34 glycoprotein on the surface of three independently derived mouse CD34-transfected cell lines. RAM34 antibody also reacts with the mouse cell lines PA6, 416B, Swiss 3T6, NIH, 3T3, DA1, and M1, all of which are positive for expression of mouse CD34 mRNA. Cell lines shown to be negative for CD34 transcript, including WEHI-3B, EL4, 18.8, and CMT64/61, are also negative for surface expression of CD34 as determined by RAM34 staining. Normal thymocytes and splenocytes are negative for CD34 expression. In the bone marrow, 7-10% of cells are stained with RAM34 mAb, including most of the Ly-6A/E (Sca-1)+ CD90 (Thy-1)low Lineage Marker- hematopoietic stem cell-enriched subpopulation and myeloerythroid progenitors. CD34 is also expressed on a small percentage of fetal liver cells, including NK-cell progenitors. CD34 has been reported to be expressed on the endothelium of capillaries and, in this form, to function as a ligand for L-selectin. Consistent with this observation, RAM34 antibody stains endothelial cells in spleen, thymus, and postcapillary HEVs in the lymph nodes. It is reported that RAM34 antibody can be used to select CD34+ CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker- bone marrow-derived hematopoietic stem cells, capable of short-term multi-lineage reconstitution of lethally irradiated mice; while the CD34- CD117+ Sca-1+ Lineage Marker- population contains self-renewing hematopoietic stem cells. Similarly, the bone marrow population with high dye-efflux capacity and which is highly enriched for long-term reconstituting hematopoietic stem cells is CD34- CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker-.
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