The Rat Aifm1 (Apoptosis-inducing factor 1, mitochondrial) ELISA Kit is specifically designed for the precise measurement of Aifm1 levels in rat serum, plasma, and tissue lysates. With its high sensitivity and specificity, this kit offers accurate and reliable results for a variety of research applications. Aifm1 protein is a mitochondrial flavoprotein that plays a crucial role in both mitochondrial function and apoptosis. In normal conditions, Aifm1 is located in the mitochondrial intermembrane space and participates in oxidative phosphorylation and mitochondrial respiration. Upon severe cellular stress (e. g., oxidative stress, DNA damage), Aifm1 translocates to the nucleus, where it induces caspase-independent apoptosis by causing chromatin condensation and large-scale DNA fragmentation.
By measuring Aifm1 levels, researchers can gain valuable insights into multiple cell process such as mitochondrial respiration, programmed cell death (caspase-independent apoptosis), and various human diseases . The study of Aifm1 is essential in fields such as neuroscience, oncology, cardiology, and rare diseases due to its central role in mitochondrial homeostasis and apoptosis. Understanding its function could help develop new therapeutic strategies for mitochondrial diseases, neurodegenerative disorders, and cancer.
Product Name:
Rat AIF1 ELISA Kit
SKU:
RTFI00292
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
88-104%
86-92%
82-97%
EDTA Plasma (n = 5)
92-105%
82-100%
87-100%
Heparin Plasma (n = 5)
86-105%
88-98%
80-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-100
91
EDTA Plasma (n = 5)
87-105
99
Heparin Plasma (n = 5)
87-105
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
