Anti-APTX Antibody (CAB5364)

Antibody Name:	Anti-APTX Antibody
Antibody SKU:	CAB5364
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:20 - 1:50
Reactivity:	Human
Positive Samples:	22Rv1, A-549, K-562, BT-474, U-87MG, HT-29, Raji

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LYPY IVEF EEEA KNPG LETH RKRK RSGN SDSI ERDA AQEA EAGT GLEP GSNS GQCS VPLK KGKD APIK KESL GHWS QGLK ISMQ DPKM QVYK DEQV VVIK DKYP KARY HWLV LPWT SISS LKAV AREH LELL KHMH TVGE KVIV DFAG SSKL RFRL GYHA IPSM SHVH LHVI SQDF DSPC LKNK KHWN SFNT EYFL ESQA VIEM VQEA GRVT VRDG MPEL LKLP LRCH ECQQ LLPS IPQL KEHL RKHW TQ
Gene ID:	54840
Uniprot:	Q7Z2E3
Cellular Location:	Cytoplasm, Nucleus, nucleolus, nucleoplasm
Calculated MW:	5kDa/13kDa/19-40kDa
Observed MW:	40kDa

Synonyms:	APTX, AOA, AOA1, AXA1, EAOH, EOAHA, FHA-HIT, aprataxin
Background:	This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.

UniProt Protein Function:	APTX: DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non- ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'- monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Defects in APTX are the cause of ataxia-oculomotor apraxia syndrome (AOA). AOA is an autosomal recessive syndrome characterized by early-onset cerebellar ataxia, oculomotor apraxia, early areflexia and late peripheral neuropathy. 13 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nucleolus; EC 3.-.-.-; C2H2-type zinc finger protein; RNA-binding; Phosphatase (non-protein); DNA repair, damage Chromosomal Location of Human Ortholog: 9p13.3 Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; nucleolus; chromatin; nucleus Molecular Function:protein binding; DNA 5'-adenosine monophosphate hydrolase activity; phosphoglycolate phosphatase activity; polynucleotide 3'-phosphatase activity; metal ion binding; double-stranded DNA binding; double-stranded RNA binding; damaged DNA binding; phosphoprotein binding; protein N-terminus binding; chromatin binding Biological Process: single strand break repair; response to hydrogen peroxide; dephosphorylation; double-strand break repair; regulation of protein stability; DNA catabolic process, exonucleolytic; response to DNA damage stimulus; DNA ligation Disease: Ataxia, Early-onset, With Oculomotor Apraxia And Hypoalbuminemia
NCBI Summary:	This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.[provided by RefSeq, Aug 2010]
UniProt Code:	Q7Z2E3
NCBI GenInfo Identifier:	48428038
NCBI Gene ID:	54840
NCBI Accession:	Q7Z2E3.2
UniProt Secondary Accession:	Q7Z2E3,Q0P662, Q5T781, Q5T782, Q5T784, Q6JV81, Q6JV82 Q6JV85, Q7Z2F3, A8MTN4, D3DRK9, D3DRL0,
UniProt Related Accession:	Q7Z2E3
Molecular Weight:
NCBI Full Name:	Aprataxin
NCBI Synonym Full Names:	aprataxin
NCBI Official Symbol:	APTX
NCBI Official Synonym Symbols:	AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT
NCBI Protein Information:	aprataxin; forkhead-associated domain histidine triad-like protein
UniProt Protein Name:	Aprataxin
UniProt Synonym Protein Names:	Forkhead-associated domain histidine triad-like protein; FHA-HIT
Protein Family:	Aprataxin
UniProt Gene Name:	APTX
UniProt Entry Name:	APTX_HUMAN

	Western blot analysis of extracts of various cell lines, using APTX antibody (CAB5364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of A549 cells using APTX antibody (CAB5364).
	Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug APTX antibody (CAB5364). Western blot was performed from the immunoprecipitate using APTX antibody (CAB5364) at a dilition of 1:500.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)