The AQP5 Antibody (CAB9927) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, meticulously raised in rabbits, demonstrates high reactivity with human samples and has been rigorously validated for Western blot applications.Aquaporin-5 is known for its involvement in maintaining water balance in the body and its dysregulation has been linked to conditions such as dry mouth, Sjogren's syndrome, and pulmonary edema. By utilizing the Aquaporin-5 Polyclonal Antibody, researchers can effectively detect and analyze Aquaporin-5 expression in different cell types, providing valuable insights into the mechanisms of water transport and its implications in various physiological and pathological processes.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
AQP5 Antibody
SKU:
CAB9927
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RSFG PAVV MNRF SPAH
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:1000 - 1:5000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13.
Purification Method
Affinity purification
Gene ID
362
RRID
AB_2768408
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using AQP5 Rabbit pAb (CAB9927) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of NIH/3T3 cells using AQP5 Rabbit pAb (CAB9927) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
