Western blot analysis of extracts of various cell lines, using ARF1 antibody (CAB21474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The ARF1 Polyclonal Antibody (CAB21474) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting ARF1 in human samples, making it ideal for use in Western blot applications.ARF1 plays a crucial role in intracellular vesicular trafficking, particularly in the Golgi apparatus and endocytic pathways. By targeting ARF1 with this polyclonal antibody, researchers can investigate its role in processes such as membrane budding, vesicle formation, and protein sorting.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ARF1 Polyclonal Antibody
SKU:
CAB21474
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
375
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ARF1 Rabbit pAb (CAB21474) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
![Anti-ARF1 [R58-6M-8] Monoclonal Antibody (AGMB03333)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/274622/679184/anti-arf1-r58-6m-8-monoclonal-antibody-agmb03333__55642.1773038077.jpg?c=2 "Anti-ARF1 [R58-6M-8] Monoclonal Antibody (AGMB03333)")
