Anti-ARL13B Antibody (CAB5200)

Antibody Name:	Anti-ARL13B Antibody
Antibody SKU:	CAB5200
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ARL13B (NP_878899.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SKOV3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ARL13B (NP_878899.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFSL MASC CGWF KRWR EPVR KVTL LMVG LDNA GKTA TAKG IQGE YPED VAPT VGFS KINL RQGK FEVT IFDL GGGI RIRG IWKN YYAE SYGV IFVV DSSD EERM EETK EAMS EMLR HPRI SGKP ILVL ANKQ DKEG ALGE ADVI ECLS LEKL VNEH KCLC QIEP CSAI SGYG KKID KSIK KGLY WLLH VIAR DFDA LNER IQKE TTEQ RALE EQEK QERA ERVR KLRE ERKQ NEQE QAEL
Gene ID:	200894
Uniprot:	Q3SXY8
Cellular Location:	Cell projection, Lipid-anchor, cilium membrane
Calculated MW:	36kDa/37kDa/48kDa
Observed MW:	49kDa

Synonyms:

ARL13B, ARL2L1, JBTS8

Background:

This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in this gene are the cause of Joubert syndrome 8. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	ARL13B: Defects in ARL13B are the cause of Joubert syndrome type 8 (JBTS8). JBTS is an autosomal recessive disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermis hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. Belongs to the small GTPase superfamily. Arf family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:G protein, monomeric; G protein, monomeric, ARF Chromosomal Location of Human Ortholog: 3q11.1 Cellular Component: intracellular; cilium Molecular Function:protein binding; GTP binding Biological Process: formation of radial glial scaffolds; smoothened signaling pathway; dorsal/ventral pattern formation; interneuron migration from the subpallium to the cortex; sensory cilium biogenesis; organelle organization and biogenesis; small GTPase mediated signal transduction; neural tube patterning; cilium biogenesis; heart looping Disease: Joubert Syndrome 8
NCBI Summary:	This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in this gene are the cause of Joubert syndrome 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
UniProt Code:	Q3SXY8
NCBI GenInfo Identifier:	115503786
NCBI Gene ID:	200894
NCBI Accession:	Q3SXY8.1
UniProt Secondary Accession:	Q3SXY8,Q504W8, Q8TCL5, D3DN29, G3V1S8,
UniProt Related Accession:	Q3SXY8
Molecular Weight:	Observed: 49kDaCalculated: 36kDa/37kDa/48kDa
NCBI Full Name:	ADP-ribosylation factor-like protein 13B
NCBI Synonym Full Names:	ADP-ribosylation factor-like 13B
NCBI Official Symbol:	ARL13B
NCBI Official Synonym Symbols:	JBTS8; ARL2L1
NCBI Protein Information:	ADP-ribosylation factor-like protein 13B; ARL2-like protein 1; ADP-ribosylation factor-like 2-like 1
UniProt Protein Name:	ADP-ribosylation factor-like protein 13B
UniProt Synonym Protein Names:	ADP-ribosylation factor-like protein 2-like 1; ARL2-like protein 1
UniProt Gene Name:	ARL13B
UniProt Entry Name:	AR13B_HUMAN

	Western blot analysis of extracts of SKOV3 cells, using ARL13B antibody (CAB5200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of C6 cells using ARL13B antibody (CAB5200) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using ARL13B antibody (CAB5200) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using ARL13B antibody (CAB5200) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

ARL13B Antibody (PACO07839)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)