Anti-ASNS Antibody (CAB16035)

Antibody Name:	Anti-ASNS Antibody
Antibody SKU:	CAB16035
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 262-561 of human ASNS (NP_597680.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SGC-7901, U-87MG, Mouse brain, Mouse pancreas, Mouse spleen, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 262-561 of human ASNS (NP_597680.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DSSL VAAT LLKQ LKEA QVQY PLQT FAIG MEDS PDLL AARK VADH IGSE HYEV LFNS EEGI QALD EVIF SLET YDIT TVRA SVGM YLIS KYIR KNTD SVVI FSGE GSDE LTQG YIYF HKAP SPEK AEEE SERL LREL YLFD VLRA DRTT AAHG LELR VPFL DHRF SSYY LSLP PEMR IPKN GIEK HLLR ETFE DSNL IPKE ILWR PKEA FSDG ITSV KNSW FKIL QEYV EHQV DDAM MANA AQKF PFNT PKTK EGYY YRQV FERH YPGR ADWL SHYW MPKW INAT DPSA RTLT HYKS AVKA
Gene ID:	440
Uniprot:	P08243
Cellular Location:
Calculated MW:	54kDa/62kDa/64kDa
Observed MW:	55, 64kDa

Synonyms:	ASNS, ASNSD, TS11
Background:	The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene.

UniProt Protein Function:	ASNS: 3 isoforms of the human protein are produced by alternative splicing
UniProt Protein Details:	Protein type:Amino Acid Metabolism - alanine, aspartate and glutamate; Cell cycle regulation; Ligase; Energy Metabolism - nitrogen; EC 6.3.5.4 Chromosomal Location of Human Ortholog: 7q21.3 Cellular Component: cytosol Molecular Function:protein homodimerization activity; asparagine synthase (glutamine-hydrolyzing) activity; cofactor binding; ATP binding Biological Process: response to light stimulus; unfolded protein response; response to toxin; glutamine metabolic process; liver development; positive regulation of mitotic cell cycle; response to amino acid stimulus; cellular response to hormone stimulus; cellular response to glucose starvation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; asparagine biosynthetic process; response to mechanical stimulus; response to methotrexate; amino acid biosynthetic process; response to follicle-stimulating hormone stimulus; negative regulation of apoptosis Disease: Asparagine Synthetase Deficiency
NCBI Summary:	The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
UniProt Code:	P08243
NCBI GenInfo Identifier:	13432102
NCBI Gene ID:	440
NCBI Accession:	P08243.4
UniProt Secondary Accession:	P08243,P08184, Q15666, Q549T9, Q96HD0, A4D1I8, B4DXZ1 B7ZAA9, D6W5R3, E9PCI3, E9PCX6,
UniProt Related Accession:	P08243
Molecular Weight:	561
NCBI Full Name:	Asparagine synthetase
NCBI Synonym Full Names:	asparagine synthetase (glutamine-hydrolyzing)
NCBI Official Symbol:	ASNS
NCBI Official Synonym Symbols:	TS11; ASNSD
NCBI Protein Information:	asparagine synthetase [glutamine-hydrolyzing]; asparagine synthetase [glutamine-hydrolyzing]; TS11 cell cycle control protein; glutamine-dependent asparagine synthetase
UniProt Protein Name:	Asparagine synthetase [glutamine-hydrolyzing]
UniProt Synonym Protein Names:	Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase
Protein Family:	Asparagine--tRNA ligase
UniProt Gene Name:	ASNS
UniProt Entry Name:	ASNS_HUMAN

	Western blot analysis of extracts of various cell lines, using ASNS antibody (CAB16035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded rat ovary using ASNS antibody (CAB16035) at dilution of 1:100 (40x lens).

Antibodies

Anti-ASNS Antibody (CAB5558)

Anti-ASNS Antibody (CAB1030)

ASNS Antibody (PACO07887)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)