The ASS1 Monoclonal Antibody (CAB9084) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbit monoclonal technology, is specifically designed for use in various research applications including immunofluorescence, immunohistochemistry, and flow cytometry.ASS1, also known as argininosuccinate synthase 1, plays a key role in the conversion of citrulline and aspartate to argininosuccinate in the urea cycle, ultimately leading to the production of urea. Dysregulation of ASS1 has been linked to a variety of diseases, including cancer and metabolic disorders, making it a valuable target for research aimed at developing new therapeutic interventions.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ASS1 Monoclonal Antibody
SKU:
CAB9084
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1407
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
445
RRID
AB_2863653
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ASS1 Rabbit mAb (CAB9084) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Confocal imaging of paraffin-embedded Human breast cancer tissue using ASS1 Rabbit mAb (CAB9084, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Mouse kidney tissue using ASS1 Rabbit mAb (CAB9084, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
