Description
| Product Name: | AST Fluorometric Activity Assay Kit |
| Product Code: | MAES0253 |
| Sample Type: | Cell, Animal Tissue, Serum (Plasma) And Other Liquid Samples |
| Assay Time: | 80 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | AST、GOT |
| Detection Instrument: | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Detection Method: | Fluorescence method |
| Sensitivity: | 0.03 U/L |
| Detection Range: | 0.03-1.5 U/L |
| Inter-assay CV: | 0.073 |
| Intra-assay CV: | 0.022 |
AST can catalyze the substrate to produce pyruvate. Pyruvate is oxidized to produce hydrogen peroxide, which causes the fluorescent probe to produce fluoresce. The activity of AST can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Instruments Required: | Micropipette, Vortex mixer, Centrifuge |
