Western blot analysis of extracts of various lysates, using ATAD3A/ATAD3B antibody (CAB22703) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The ATAD3A/ATAD3B Polyclonal Antibody (CAB22703) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications.ATAD3A and ATAD3B are essential for maintaining mitochondrial homeostasis and are implicated in various cellular processes such as mitochondrial nucleoid organization, lipid metabolism, and mitochondrial fusion. Dysregulation of these proteins has been linked to mitochondrial dysfunction and diseases like neurodegenerative disorders and metabolic syndromes.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ATAD3A/ATAD3B Polyclonal Antibody
SKU:
CAB22703
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is localized to the mitochondrial inner membrane, where it can bind to a highly-related protein, ATAD3A. ATAD3A appears to interact with matrix nucleoid complexes, and the encoded protein negatively regulates that interaction. This gene is expressed almost exclusively in pluripotent embryonic stem cells and some cancer cells. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
55210 83858
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ATAD3A/ATAD3B Rabbit pAb (CAB22703) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of U20S cells using ATAD3A/ATAD3B Rabbit pAb (CAB22703) at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of H9C2 cells using ATAD3A/ATAD3B Rabbit pAb (CAB22703) at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
