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Autophagy Antibodies Anti-ATG3 Antibody CAB19594
Key Features

ATG3 Rabbit Monoclonal Antibody (CAB19594)

Datasheet MSDS
SKU CAB19594
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms APG3, APG3L, hApg3, PC3-96, APG3-LIKE, ATG3
Research Use Autophagy
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

ATG3 Monoclonal Antibody (CAB19594)

The ATG3 Monoclonal Antibody (CAB19594) is a high-quality antibody developed for reliable detection and analysis of target proteins. This highly specific antibody, generated from rabbit immunizations, is optimized for detecting and analyzing ATG3 protein levels in a variety of samples, but is particularly effective in human samples.ATG3 is a crucial component of the autophagy pathway, responsible for conjugating ATG8-like proteins to phosphatidylethanolamine, facilitating the formation of autophagosomes. Dysregulation of autophagy has been implicated in various diseases, including neurodegenerative disorders, cancer, and infectious diseases.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ATG3 Monoclonal Antibody
SKU:CAB19594
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC0073
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MQNV INTV KGKA LEVA EYLT PVLK ESKF KETG VITP EEFV AAGD HLVH HCPT WQWA TGEE LKVK AYLP TGKQ FLVT KNVP CYKR CKQM EYSD ELEA IIEE
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:1000 - 1:2000
IHC-P1:200 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:APG3, APG3L, hApg3, PC3-96, APG3-LIKE, ATG3
Positive Sample:Jurkat, U-87 MG, K-562, Mouse brain, Mouse testis, Rat testis
Cellular Localization:Cytoplasm.
Calculated MW:40kDa
Observed MW:40kDa

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.

Purification MethodAffinity purification
Gene ID64422
RRIDAB_2862687
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using ATG3 Rabbit mAb (CAB19594) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 40s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human kidney tissue using ATG3 Rabbit mAb (CAB19594) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human placenta tissue using ATG3 Rabbit mAb (CAB19594) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ATG3 Rabbit mAb (CAB19594) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat liver tissue using ATG3 Rabbit mAb (CAB19594) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ATG3 Rabbit mAb (CAB19594) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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