Western blot analysis of extracts of various cell lines, using ATG4D antibody (CAB21690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The ATG4D Polyclonal Antibody (CAB21690) is a high-quality antibody developed for reliable detection and analysis of target proteins. Autophagy is a cellular mechanism essential for maintaining cellular homeostasis and is implicated in various diseases such as cancer, neurodegenerative disorders, and infections.This antibody, generated in rabbits, specifically targets the ATG4D protein and is highly reactive with human samples. It has been validated for use in Western blot applications, allowing for the detection and analysis of ATG4D in various cell types. This makes it an ideal choice for studies in the fields of cell biology, cancer research, and infectious diseases.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ATG4D Polyclonal Antibody
SKU:
CAB21690
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
APG4D, AUTL4, APG4-D, HsAPG4D, ATG4D
Positive Sample:
22Rv1, NCI-H460, Mouse heart
Cellular Localization:
Cytoplasm, Mitochondrion Matrix.
Calculated MW:
53kDa
Observed MW:
Refertofigures
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
84971
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ATG4D Rabbit pAb (CAB21690) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using ATG4D Rabbit pAb (CAB21690) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
