Anti-ATP5A1 Antibody (CAB5884)

Antibody Name:	Anti-ATP5A1 Antibody
Antibody SKU:	CAB5884
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP5A1 (NP_004037.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT474, SKOV3, HeLa, MCF-7, Mouse kidney, Mouse heart, Rat kidney, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP5A1 (NP_004037.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLSV RVAA AVVR ALPR RAGL VSRN ALGS SFIA ARNF HASN THLQ KTGT AEMS SILE ERIL GADT SVDL EETG RVLS IGDG IARV HGLR NVQA EEMV EFSS GLKG MSLN LEPD NVGV VVFG NDKL IKEG DIVK RTGA IVDV PVGE ELLG RVVD ALGN AIDG KGPI GSKT RRRV GLKA PGII PRIS VREP MQTG IKAV DSLV PIGR GQRE LIIG DRQT GKTS IAID TIIN QKRF NDGS DEKK KLYC IYVA IGQK RSTV AQLV KRLT DADA MKYT IVVS ATAS
Gene ID:	498
Uniprot:	P25705
Cellular Location:	Cell membrane, Extracellular side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:	54kDa/57kDa/59kDa
Observed MW:	53kDa

Synonyms:

ATP5A1, ATP5A, ATP5AL2, ATPM, COXPD22, HEL-S-123m, MC5DN4, MOM2, OMR, ORM, hATP1

Background:

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

UniProt Protein Function:	ATP5A1: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. Belongs to the ATPase alpha/beta chains family.
UniProt Protein Details:	Protein type:EC 3.6.3.14; Transporter; Mitochondrial; Hydrolase; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 18q21 Cellular Component: membrane; mitochondrial inner membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex; mitochondrion; plasma membrane; signalosome Molecular Function:ATP binding; ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; MHC class I protein binding; protein binding; transmembrane transporter activity Biological Process: ATP biosynthetic process; embryonic development; lipid metabolic process; mitochondrial ATP synthesis coupled proton transport; negative regulation of endothelial cell proliferation Disease: Combined Oxidative Phosphorylation Deficiency 22; Mitochondrial Complex V (atp Synthase) Deficiency, Nuclear Type 4
NCBI Summary:	This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012]
UniProt Code:	P25705
NCBI GenInfo Identifier:	114517
NCBI Gene ID:	498
NCBI Accession:	P25705.1
UniProt Secondary Accession:	P25705,Q53XX6, Q8IXV2, Q96FB4, Q96HW2, Q96IR6, Q9BTV8 A8K092, B4DY56, K7ENP3,
UniProt Related Accession:	P25705
Molecular Weight:	57,547 Da
NCBI Full Name:	ATP synthase subunit alpha, mitochondrial
NCBI Synonym Full Names:	ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
NCBI Official Symbol:	ATP5A1
NCBI Official Synonym Symbols:	OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m
NCBI Protein Information:	ATP synthase subunit alpha, mitochondrial
UniProt Protein Name:	ATP synthase subunit alpha, mitochondrial
UniProt Gene Name:	ATP5A1
UniProt Entry Name:	ATPA_HUMAN

	Western blot analysis of extracts of various cell lines, using ATP5A1 Antibody (CAB5884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat ovary using ATP5A1 antibody (CAB5884) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using ATP5A1 antibody (CAB5884) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using ATP5A1 antibody (CAB5884) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using ATP5A1 antibody (CAB5884) at dilution of 1:100 (40x lens).

Antibodies

Anti-ATP5A1 Antibody (CAB11217)

ATP5A1 Antibody (PACO06270)

ATP5A1 Antibody (PACO07923)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)