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Anti-ATP6V0C Antibody (CAB16350)

SKU:
CAB16350
Product Type:
Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
  • Immunology Antibodies 2 Anti-ATP6V0C Antibody CAB16350
  • Immunology Antibodies 2 Anti-ATP6V0C Antibody CAB16350
€139 - €419
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Description

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Antibody Name:Anti-ATP6V0C Antibody
Antibody SKU:CAB16350
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATP6V0C (NP_001185498.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Mouse, Rat
Positive Samples:Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATP6V0C (NP_001185498.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSES KSGP EYAS FFAV MGAS AAMV FSAL GAAY GTAK SGTG IAAM SVMR PEQI MKSI IPVV MAGI IAIY GLVV AVLI ANSL NDDI SLYK SFLQ LGAG LSVG
Gene ID:527
Uniprot:P27449
Cellular Location:Multi-pass membrane protein, Vacuole membrane
Calculated MW:15kDa
Observed MW:16kDa
Synonyms:ATP6V0C, ATP6C, ATP6L, ATPL, VATL, VPPC, Vma3
Background:This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. This gene encodes the V0 subunit c. Alternative splicing results in transcript variants. Pseudogenes have been identified on chromosomes 6 and 17.
UniProt Protein Function:ATP6V0C: Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase proteolipid subunit family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass; Transporter, ion channel; Transporter, iron; Transporter

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: phagocytic vesicle membrane; focal adhesion; lysosomal membrane; integral to membrane; endosome membrane

Molecular Function:protein binding; ubiquitin protein ligase binding; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrogen ion transporting ATPase activity, rotational mechanism

Biological Process: interaction with host; proton transport; viral reproduction; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport; positive regulation of Wnt receptor signaling pathway

NCBI Summary:This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. This gene encodes the V0 subunit c. Alternative splicing results in transcript variants. Pseudogenes have been identified on chromosomes 6 and 17. [provided by RefSeq, Nov 2010]
UniProt Code:P27449
NCBI GenInfo Identifier:310832382
NCBI Gene ID:527
NCBI Accession:NP_001185498.1
UniProt Secondary Accession:P27449,Q6FH26,
UniProt Related Accession:P27449
Molecular Weight:15,736 Da
NCBI Full Name:V-type proton ATPase 16 kDa proteolipid subunit
NCBI Synonym Full Names:ATPase, H+ transporting, lysosomal 16kDa, V0 subunit c
NCBI Official Symbol:ATP6V0C  
NCBI Official Synonym Symbols:ATPL; VATL; VPPC; Vma3; ATP6C; ATP6L  
NCBI Protein Information:V-type proton ATPase 16 kDa proteolipid subunit; V-ATPase 16 kDa proteolipid subunit; vacuolar H+ ATPase proton channel subunit; vacuolar proton pump 16 kDa proteolipid subunit; vacuolar ATP synthase 16 kDa proteolipid subunit; H(+)-transporting two-sector ATPase, 16 kDa subunit
UniProt Protein Name:V-type proton ATPase 16 kDa proteolipid subunit
UniProt Synonym Protein Names:Vacuolar proton pump 16 kDa proteolipid subunit
UniProt Gene Name:ATP6V0C  
UniProt Entry Name:VATL_HUMAN
Anti-ATP6V0C Antibody (CAB16350)Western blot analysis of extracts of various cell lines, using ATP6V0C Rabbit pAb (CAB16350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-ATP6V0C Antibody (CAB16350)Immunofluorescence analysis of C6 cells using ATP6V0C Polyclonal Antibody (CAB16350) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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