Anti-ATRIP Antibody (CAB7139)

Antibody Name:	Anti-ATRIP Antibody
Antibody SKU:	CAB7139
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ATRIP (NP_569055.1).

Application:	WB IP
Recommended Dilution:	WB 1:500 - 1:2000 IP 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HepG2, Jurkat, HeLa, Mouse brain, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ATRIP (NP_569055.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGT SAPG SKRR SEPP APRP GPPP GTGH PPSK RARG FSAA AAPD PDDP FGAH GDFT ADDL EELD TLAS QALS QCPA AARD VSSD HKVH RLLD GMSK NPSG KNRE TVPI KDNF ELEV LQAQ YKEL KEKM KVME EEVL IKNG EIKI LRDS LHQT ESVL EEQR RSHF LLEQ EKTQ ALSD KEKE FSKK LQSL QSEL QFKD AEMN ELRT KLQT SERA NKLA APSV SHVS PRKN PSVV IKPE ACSP QFGK TSFP TKES FSAN MSLP
Gene ID:	84126
Uniprot:	Q8WXE1
Cellular Location:	Nucleus
Calculated MW:	72kDa/76kDa/83kDa/85kDa
Observed MW:	86kDa

Synonyms:

ATRIP

Background:

This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ATRIP: is required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein. Heterodimerizes with ATR. The heterodimer binds replication protein A (RPA), a protein complex that subsequently is recruited to single stranded DNA (ssDNA). Its EEXXXDDL motif is required for the interaction with catalytic subunit of DNA-PK and its recruitment to sites of DNA damage. e for this protein is either identical to or adjacent to that of ATRIP. Some of the mRNAs that encode ATRIP also encode TREX1 in another reading frame. Three alternatively spliced human isoforms have been reported.
UniProt Protein Details:	Protein type:DNA-binding; DNA repair, damage; Cell cycle regulation Chromosomal Location of Human Ortholog: 3p21.31 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding Biological Process: DNA damage checkpoint; DNA repair; DNA replication
NCBI Summary:	This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012]
UniProt Code:	Q8WXE1
NCBI GenInfo Identifier:	48428109
NCBI Gene ID:	84126
NCBI Accession:	Q8WXE1.1
UniProt Secondary Accession:	Q8WXE1,Q69YK9, Q8NHQ2, Q8WUG7, Q96CL3, Q9HA30, A8K6A3 A8K714, B2RCE7, B4DU92, B5MEB7,
UniProt Related Accession:	Q8WXE1
Molecular Weight:	791
NCBI Full Name:	ATR-interacting protein
NCBI Synonym Full Names:	ATR interacting protein
NCBI Official Symbol:	ATRIP
NCBI Protein Information:	ATR-interacting protein; ATM and Rad3-related-interacting protein
UniProt Protein Name:	ATR-interacting protein
UniProt Synonym Protein Names:	ATM and Rad3-related-interacting protein
Protein Family:	ATR-interacting protein
UniProt Gene Name:	ATRIP
UniProt Entry Name:	ATRIP_HUMAN

	Western blot analysis of extracts of various cell lines, using ATRIP antibody (CAB7139) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ATRIP antibody (CAB7139). Western blot was performed from the immunoprecipitate using ATRIP antibody (CAB7139) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-ATRIP Antibody (CAB5041)	ATRIP Colorimetric Cell-Based ELISA Kit
Phospho-ATRIP (S68) Antibody (PACO03151)	ATRIP (Phospho-Ser68) Colorimetric Cell-Based ELISA Kit
Phospho-ATRIP (S224) Antibody (PACO05496)	ATRIP (Phospho-Ser224) Colorimetric Cell-Based ELISA Kit
ATRIP Antibody (PACO05497)
ATRIP Antibody (PACO06272)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)