Anti-AVPR1A Antibody (CAB8400)

Antibody Name:	Anti-AVPR1A Antibody
Antibody SKU:	CAB8400
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 349-418 of human AVPR1A (NP_000697.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, A-549, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 349-418 of human AVPR1A (NP_000697.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFFS GHLL QDCV QSFP CCQN MKEK FNKE DTDS MSRR QTFY SNNR SPTN STGM WKDS PKSS KSIK FIPV ST
Gene ID:	552
Uniprot:	P37288
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	46kDa
Observed MW:	60kDa

Synonyms:

AVPR1A, AVPR V1a, AVPR1, V1aR, AVPRV1a

Background:

The protein encoded by this gene acts as receptor for arginine vasopressin. This receptor belongs to the subfamily of G-protein coupled receptors which includes AVPR1B, V2R and OXT receptors. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. The receptor mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis.

UniProt Protein Function:	AVPR1A: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl- inositol-calcium second messenger system. Has been involved in social behaviors, including affiliation and attachment. Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral Chromosomal Location of Human Ortholog: 12q14-q15 Cellular Component: endosome; integral to plasma membrane; plasma membrane Molecular Function:peptide binding; protein binding; protein kinase C binding Biological Process: blood circulation; cellular response to hormone stimulus; generation of precursor metabolites and energy; maternal behavior; phospholipase C activation; positive regulation of vasoconstriction; regulation of systemic arterial blood pressure by vasopressin
NCBI Summary:	The protein encoded by this gene acts as receptor for arginine vasopressin. This receptor belongs to the subfamily of G-protein coupled receptors which includes AVPR1B, V2R and OXT receptors. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. The receptor mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis. [provided by RefSeq, Jul 2008]
UniProt Code:	P37288
NCBI GenInfo Identifier:	586197
NCBI Gene ID:	552
NCBI Accession:	P37288.1
UniProt Related Accession:	P37288
Molecular Weight:	46,800 Da
NCBI Full Name:	Vasopressin V1a receptor
NCBI Synonym Full Names:	arginine vasopressin receptor 1A
NCBI Official Symbol:	AVPR1A
NCBI Official Synonym Symbols:	V1aR; AVPR1; AVPR V1a
NCBI Protein Information:	vasopressin V1a receptor
UniProt Protein Name:	Vasopressin V1a receptor
UniProt Synonym Protein Names:	AVPR V1a; Antidiuretic hormone receptor 1a; Vascular/hepatic-type arginine vasopressin receptor
Protein Family:	Vasopressin V1a receptor
UniProt Gene Name:	AVPR1A
UniProt Entry Name:	V1AR_HUMAN

	Western blot analysis of extracts of various cell lines, using AVPR1A antibody (CAB8400) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Immunofluorescence analysis of C6 cells using AVPR1A Rabbit pAb (CAB8400) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using AVPR1A Rabbit pAb (CAB8400) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
AVPR1A Antibody (PACO07447)	Human AVPR V1a / Vasopressin V1a receptor ELISA Kit
	Human AVPR1A (Arginine Vasopressin Receptor 1A) ELISA Kit (HUES01697)
	Human Vasopressin V1a receptor (AVPR1A) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)