Description
BDBV Nucleoprotein Recombinant Protein (N-His)
BDBV Nucleoprotein Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gln38-Glu385, with an N-terminal His tag. Predicted molecular weight: 41.16 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Nucleoprotein is a ~82 kDa protein. Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. For research use only.
VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm.
| Product Name: | BDBV Nucleoprotein Recombinant Protein (N-His) |
| SKU: | GXRP10005 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | BDBV |
| Synonyms: | Nucleoprotein, Nucleocapsid protein, NP |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gln38-Glu385 |
| Calculated MW: | 41.16 kDa |
| UniProt: | B8XCM7 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
