Biotinylated Recombinant Human CD3E&CD3D Protein (Primary Amine Labeling)
The Biotinylated Recombinant Human CD3E&CD3D Protein (Primary Amine Labeling) is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Biotinylated CD3E&CD3D (Primary Amine Labeling) function and its implications in health and disease.
This product (SKU: RPCB0669) is produced using advanced expression systems and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 17.9 kDa (CD3E),16.7 kDa (CD3D) with an observed molecular weight of 25-40 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Human CD3E&CD3D Protein (Primary Amine Labeling)
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human CD3E&CD3D Protein (Primary Amine Labeling) (RPCB0669), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D, are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Biotinylated Recombinant Human CD3E&CD3D Protein (Primary Amine Labeling) was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinylated Human CD3E&CD3D is greater than 95% as determined by SEC-HPLC.
