Biotinylated Recombinant Human Siglec-3/CD33 Protein
The Biotinylated Recombinant Human Siglec-3/CD33 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Biotinylated Siglec-3/CD33 function and its implications in health and disease.
This product (SKU: RPCB0696) is produced using advanced expression systems and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 29.6 kDa with an observed molecular weight of 48-58 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Human Siglec-3/CD33 Protein
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human Siglec-3/CD33 Protein (RP00555BLQ), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.
Formulation:
Supplied as 0.22 μm filtered solution in 50 mM MES, 500 mM NaCl, 200 mM L-arginine (pH 7.4).
Storage:
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
Biotinylated Recombinant Human Siglec-3/CD33 Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinylated Human Siglec-3 is greater than 90% as determined by SEC-HPLC.
